The event, organised by business advisers Mazars and hosted by BBC Match of the Day commentator Steve Wilson, takes place on July 5 at the DoubleTree by Hilton, Stadium MK.
It will raise money for the Winter Night Shelter MK, which runs from December to mid-March and provides hot food and a bed to up to 15 people each night.
Mazars’ office managing partner in Milton Keynes, Stephen Eames, said: "You cannot help but notice the homeless around the city. You have only to walk between the shopping centre and the station and you will encounter at least half a dozen people sleeping rough.
"This is an issue that we cannot ignore.”
The minimum donation is £100 per team, Entrants can make their entry donation via a charity choice giving page at: http://www.charitychoice.co.uk/fundraiser/lorraineconnolly/mazars-charity-quiz or by cheque. To enter, e-mail Lorraine Connolly at Lorraine.firstname.lastname@example.org
Launched five years ago, Winter Night Shelter MK funds a welfare officer to work with those affected by homelessness.